Start Denudating


Hence, the fertilizing ability of an oocyte with a mature nucleus is not necessarily at its maximum potential.

The timing between oocyte retrieval and oocytes denudation was the timing elapsed between the beginning of oocyte retrieval and the beginning of oocyte denudation (Assessment of fertilization was made 17-18 h after ICSI by checking the number of polar bodies and pronuclei.

The fertilization rate was defined as the ratio between the number of diploid zygotes and the number of mature oocytes.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. To analyze the impact of oocyte denudation and microinjection timings on intracytoplasmic sperm injection (ICSI) outcomes. We included ICSI cycles with the following parameters: rank 1 or 2, female age Intracytoplasmic sperm injection (ICSI) is the treatment of choice for couples with severe male infertility.

The microinjection technique has been completely standardized but there is no common standard for the precise timings of all the procedures.

No more than 7 studies focusing on the influence of ICSI procedure timings on the outcoming results were published [1–8], with discrepancies in the conclusions.

Although it has been shown that incubation of oocytes for 2–6 h prior to IVF (In Vitro Fertilization) improves fertilization and pregnancy rates [9–12], there are some conflicting results regarding the timing of ICSI.

Sperm injections were performed throughout the day, depending of the number of ICSI procedures, and the workload in the laboratory.