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The timing between the end of sperm preparation and the beginning of ICSI was comprised between 30 minutes and 2 hours.

There was no difference in the fertilization and implantation rates between the different operators.

However, other studies supported different results as no statistically significant differences in the fertilization [3, 4] or the pregnancy rates [2, 6] were found in preincubated oocytes during ICSI cycles.

In theory, some problems may be associated with the injection time.

During all the time of the current study, the ICSI conditions were identical (equipments, media, and culture conditions).